tDenHyb™ Solutions
(for Tissue FISH on paraffin-embedded tissuse slides)
tDenHyb™ solutions are highly effective hybridization solutions for FISH
on paraffin-embedded cells and tissue sections. tDenHyb™ solutions are compatible
with a wide range of home-brewed or commercially available directly- and indirectly
labeled DNA probes: repeat sequence, paint, and unique sequence probes.
- tDenHyb-1 is optimized for FISH on paraffin-embedded tissue sections
when repeat sequence/centromeric probes were used.
But tDenHyb-1 is less effective for unique sequence probes.
- tDenHyb-2 is optimized for FISH on paraffin-embedded tissue sections
when unique sequence probes were used.
But tDenHyb-2 is less effective for repeat sequence probes.
Dilute or suspend directly or indirectly-labeled DNA probes in an appropriate tDenHyb™ solution. Then, perform
your in-house tissue FISH procedure with the DNA probes (in tDenHyb™). More
effective and convenient FISH can be achieved by using Insitus MetalTray FISH
protocols* which are based on the use of Metal
Slide Tray and HybBox™ in conjunction with the use
of tDenHyb.
If you perform FISH with Insitus tDenHyb™ solutions using your in-house
FISH protocol or protocol provided by vendors that sell DNA probes, a slight adjustment
of your denaturation condition close to the Insitus Manual Tissue FISH protocols
would maximize hybridization signals.
If you are doing FISH with your home-brewed DNA probes, simply dilute or suspend your probes in tDenHyb™ solution. Add blocking DNA if necessary. The optimal concentration
of the home-brew probe must be determined empirically.
tDenHyb solutions can be used for the application to tissue microarrays (TMA) with DNA probes.
Available tDenHyb* are:
- Catalog # D101; tDenHyb-1, 1 ml
- Catalog # D102; tDenHyb-2, 1 ml
*Both tDenHyb-1 and tDenHyb-2
solutions are very turbid.
In
addition, tDenHyb Trial Set is available. The
set is intended for the first time users to try out both tDenHyb™ solutions
in their laboratories.
