Humid Box (for Metal Slide Tray)

This is a microwave-reheatable plastic container (approximately 5.5" x 7.3/4").   Insitus Metal Slide Tray fits to this plastic container. This container can be used conveniently as a Humid Box for overnight hybridization when Insitus Metal Slide Tray (with slides on) was used.  

Cut several paper towels to the size of the flat bottom of the plastic container. Stack up about 10 or more sheets of cut paper towels. Soak them in water and place them at the bottom of the container. Excess water can be decanted out. Put the lid on top and place the box in a 37°C incubator. This box is used as "humid box" for overnight hybridization when a metal slide tray is used. Maintain the humid box at 38°C in the incubator .

After denaturation, place the hot metal slide tray (with slides) on top of the wet paper towels in the humid box. Close the lid and leave the humid box in the incubator overnight at 38°C for hybridization.
           

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